PSA Nanobiosensor Using Graphene and Magnetic Nanoparticles
Volume Title: 1
1associate prof
2Department of Life Science Engineering, Faculty of New Science & Technology, University of Tehran, Tehran, Iran
Prostate specific antigen (PSA) is the most common biomarker of prostate cancer, which can be used to detect the prostate cancer in early stages. A novel electrochemical biosensor based on aptamer and magnetic graphene oxide/gold nanocomposite as a label-free in the ferrocyanide and methylene blue-labeled in phosphate buffer was designed. To investigate the assembly process of the nanocomposite the cyclic voltammetry (CV), Squarewave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) techniques were performed. In addition, the selectivity and specificity of the aptasensor to the PSA were examined. The result showed that the fabricated aptasensor possessed high selectivity toward the PSA in comparison to the other analytes such as glucose, fetal bovine serum (FBS), bovine serum albumin (BSA), carcinoembryonic antigen (CEA), and antibody. The limit of detection of the apatasensor was obtained 1.37 ng/mL with ferricyanide at R2 = 0.9777 and 1.57 pg/mL at R2 = 0.9676.