Study of kinetic adsorption human insulin on magnetic imprinting polymer
Volume Title: 1
Iran University of Science and Technology
In this work, Magnetic molecular imprinting polymers (MMIP) were used to separate recombinant human insulin. Different kinetic models for the adsorption of insulin on MMIPs and magnetic molecular non-imprinting polymers (MNIPs) were investigated. The results were shown that the adsorption capacity (Q, mg.g-1) of the MMIPs, and imprinting factor (IF) are found to be 66.5 mg.g-1, and 3, respectively. Studies demonstrate that the pseudo-second-order model has a better fit (R2= 0.9988) for insulin adsorption kinetics on MMIPs. Moreover, the Elovich confirmed the chemisorption adsorption of insulin on MMIPs (R2=0.998). The characterization of synthesized particles was applied by Fourier transform infrared spectrometry (FT-IR), and the vibrating sample magnetometer (VSM) and the MMIPs achieved rapid magnetic separation (19.93 emu g-1).
Fe3O4 magnetic nanoparticle; Insulin; kinetic adsorption model; Magnetic molecular imprinting polymer