Antiproliferative Effect of Fucoxanthin on Caco-2 (human colorectal carcinoma) and HepG2 (human hepatoma) Cells
عنوان دوره: 1
1School of Chemical Engineering, College of Engineering, University of Tehran, Tehran 14176, Iran
2School of Chemical Engineering
3Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Fucoxanthin is a major pigment in edible brown algae, has many properties (antioxidant, antibacterial, anticancer, antiobesity, antiinflammatory, etc.) due to its unique structure. Fucoxanthinol (fucoxanthin metabolite) has also anticancer effect. It is hydrolyzed from dietary fucoxanthin in the gastrointestinal tract before absorption in the intestine. Rcently, owing to a lot of side effects that is caused from chemical medicine used in chemotherapy, researchers attention turned to natural compound with anticancer activity e.g. fucoxanthin. The anticancer activity of fucoxanthin was assesed in the current study. To this end, microalga was cultivated in synthetic seawater enriched with f/2 medium. In the late of exponential phase the cells were harvested and three concentration of total carotenids (50, 250, 2500 µg/ml) and fucoxanthin (4, 10, 20 µg/ml) were prepared for measuring the anticancer activity on Caco-2 and HepG2 cells line through MTT [3-(4,5 dimethylthiazolyl)-2,5-diphenyl-tetrazolium bromide] assay. The results showed that total carotenoid and purified fucoxanthin at 24-hour treatment with 20 and 2500 µg/ml concentration respectively, has fairely effective anticancer activity on the Caco-2 and HepG2 cells line. By considering the attained results, chemotherapy and chemical drugs used in cancer treatment, can be replaced by purified fucoxanthin which is a hopeful supplementary diet in food and pharmaceutical industries.