Bio-Hydrogen Production Enhancement of Synechocystis sp. PCC6803 Using Systems Metabolic Engineering Approach
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1Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
2Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115- 143, Tehran, Iran.
3Department of Chemical and Petroleum Engineering, Sharif University of Technology, Azadi Avenue, P.O.Box 11365-9465, Tehran, Iran.
Bio-hydrogen production from microalgae and cyanobacteria has attracted much attention as it is renewable, reliable, and economical. The generation of bio-hydrogen from cyanobacteria can be exciting and promising options for clean energy in the future. Advances in metabolic engineering may significantly increase hydrogen production in cyanobacteria. Manipulation of the competing metabolic pathways by modulating some key enzymes such as hydrogenase and nitrogenase may seem to be a promising method of incrementing bio-hydrogen. In the present work, the metabolic pathways that have an impact on bidirectional NiFe hydrogenase which used for producing biological hydrogen in Synechocystis sp. PCC 6803 has been investigated. Therefore, the reactions such as G3PD2_b, H2ASE_syn_f, H2tpp_b, and H2tex_b are recommended to be up-regulated; however, ALCD19_f, GLYALDDr_b, GLYK, H2Otex_b are good candidates to be down-regulated.
Bio-Hydrogen; LAMOS; Genome-scale Metabolic Model; Synechocystis sp. PCC6803; Systems Metabolic Engineering