Oxidized Schizophyllan as a Non-toxic Cross-linker for Stabilizing Bovine Serum Albumin Nanogels Via Inverse Emulsion
Volume Title: 1
Department of Biotechnology, Faculty of chemical engineering, Tarbiat Modares University, P.O. BOX 14115-143, Tehran, Iran.
Nowadays using natural, nontoxic materials as a cross-linking agent has widely attracted interest for synthesizing biomaterial based nanocarriers. In this research schizophyllan (SPG), a natural water soluble exopolysaccharide was produced by cultivating Schizophyllum commune ATCC 38548, and oxidized by sodium metaperiodate (NaIO4), then used as a nontoxic, green cross-linker for stabilizing bovine serum albumin (BSA) nanogels via Schiff base reaction using inverse emulsion method. Formation of immine linkage between amino groups of BSA and aldehyde groups of SPG was confirmed by Fourier transform infrared microscopy (FTIR) analysis. Size and zeta potential were evaluated by Malvern Zetasizer 3000HS. Based on the results The obtained nanogels possess great potential for using in the biomedical applications.
Schizophyllan; Bovine serum albumin; Schizophyllum commune; Schiff base reaction; Nanogels; inverse emulsion