Synthesis and characterization of Fe3O4@SiO2 core/shell nanoparticles for biological applications
Volume Title: 1
Department of Materials Engineering, Tarbiat Modares University, P.O. Box 14115-143, Tehran, Iran
Magnetic nanoparticles have received extensive attention in biological applications such as enrichment, bioseparation, detection, and purification. In this report, nanoparticles of magnetite (Fe3O4) are prepared from the solution of ferrous/ferric mixed salt solution in an alkaline solution using the modified chemical coprecipitation process. Next, the synthesized magnetic nanoparticles were coated with silica shells by the Stober method. Then, the surface of Fe3O4@SiO2 nanoparticles modified with organosilane for the investigation of biological applications. The core/shell nanoparticles were characterized by X-ray powder diffraction (XRD), Fourier transforms infrared spectrometer (FTIR) ,and scanning electron microscopy (SEM). The results of XRD indicated that the nanoparticles were composed of crystalline Fe3O4 core and SiO2 shell. The SEM image of Fe3O4 nanoparticles showed the diameter could be tuned from 15 to 25 nm. The average diameter of the uniform spherical core/shell nanoparticles was approximately 170 ± 50 nm, and the average thickness of the silica shell was 30 nm. Finally, the protein adsorption studies on the nanoparticles revealed the ability of these nanoparticles for use in biological applications.