Systems Metabolic Engineering Approach To Increase Ethanol Production Of Synechocystis sp. PCC 6803
Volume Title: 1
Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115- 143, Tehran, Iran.
Cyanobacteria are the only prokaryotes capable of oxygen photosynthesis. Their evolutionary association with plastids in eukaryotic phototrophs and their growing use as green cell factories prompted new approaches to the use of systems biology. Synechocystis sp. PCC 6803 has become one of the most commonly used models for genetic tools in cyanobacteria. In this research, we have employed a systems metabolic engineering strategy for screening genes in order to identify down-regulated or over-expressed reactions for increasing ethanol production. Glyoxylate Carboligase (GLXCL), Fructose-bisphosphate Aldolase (FBA_b), and Aldehyde dehydrogenase (ALDD2xr_b) are ideal candidates to be up-regulated and Glycine Cleavage System (GLYCLb, GLYCLc_f), and Cytochrome b6/f complex periplasm (CBFCpc) to be down-regulated. By this approach, much time and expenses can be saved for researchers to achieve maximal ethanol production through genetic modification of Synechocystis sp. PCC 6803.