Examination of high voltage on immobilized microbial cell by electrospraying technique: Bead size and cell viability
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1Department of Environment, College of Environment, Karaj, Iran
2Faculty of New Technologies Engineering, Zirab campus, Shahid Beheshti University, Tehran, Iran
Immobilization of enzymes and viable bacterial cells has a specific area of application in food industry and biomedical sciences. Encapsulation of cells in alginate spherical hydrogel beads is one of the most common and effective immobilization techniques. Alginate bead size is an essential and effective factor in biomedical and biological applications. Electrospraying technique is a successful technology in generation of hydrogel beads with small and uniform sizes in which microcapsules are produced by applying high voltages. Since the high voltage is most important factor to prepare microcapsules, in this regard we investigated effect of various voltages (0, 5 and 10 kV) on micro-beads size as well as microbial cells viability. The results indicate that the bead size decreases with voltage increment, and there is a greater reduction at higher voltages, so that microparticles can be obtained. Also, applied high voltage has partially adverse effect on the cell viability. Therefore, we concluded that immobilized cell on alginate beads by electrospraying technique can be improved mass transfer due to small size of beads with negligible loss of cell viability.
Alginate micro-beads; Bacillus licheniformis; Electrospraying technique; immobilization; Microencapsulation