Study on reduction of polydispersity of hyaluronic acid produced by recombinant Corynebacterium glutamicum
Volume Title: 1
Department of Industrial Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB)
Hyaluronic acid (HA) is a native, linear polymer consisting of repeating units of N-acetyl glucosamine and d-glucuronic acid. This polymer is widely used in medical and cosmetic fields. HA application is strongly dependent to the molecular weight of this polymer. In this study, recombinant Corynebacterium glutamicum was employed for HA production. The gel electrophoresis assay showed a smear of polysaccharide, so it could be concluded that a wide range of HA molecular weight (MW) has been produced. The effect of carbon and nitrogen sources on HA molecular weight distribution was investigated. The results indicated that while nitrogen source substituted from soy protein to corn syrup, the molecular weight dispersion decreased, and HA production increased under similar conditions.