Protein-based hybrid scaffold as a controlled release system for skin tissue engineering application
Volume Title: 1
1مهندسی شیمی- دانشگاه صنعتی شریف
2مهندسی شیمی دانشگاه صنعتی شریف
Fabrication of skin substitutes to accomplish both aesthetic and functional demands is vital for patients with acute and chronic wounds as well as burn injuries. Accordingly, this work aimed to prepare a new silk fibroin–gelatin (SF–G) hybrid scaffold as a controlled release system and a transplantation vehicle for seeded adipose-derived mesenchymal stem cells (AMSCs). The effect of SF: G fibers volume ratio on the different properties of SF–G hybrid scaffolds were evaluated. Physical, mechanical, and biological characterization of the prepared scaffolds demonstrated that physical and mechanical properties of SF–G fibrous scaffolds enhanced significantly and a suitable SF: G fibers volume ratio for further investigation is 60:40. Moreover, the release study of bovine serum albumin (BSA) and simvastatin (SIM)-loaded scaffolds indicated that in addition to the physicochemical properties of these two substances, the type of fiber and hybrid structure of the scaffold has a vital role in their release behavior. Also, the cell analysis exhibited suitable biocompatibility of SF–G hybrid scaffolds and promising potential of these fibrous scaffolds as new candidates in biomedical engineering applications.
Adipose-derived mesenchymal stem cell; Bio-hybrid; Co-electrospinning; Drug delivery; Scaffold; Tissue engineering