Alginate-based hydrogel particles as a gastrointestinal-resistant carrier for probiotic bacteria
Volume Title: 1
1Biotechnology group, Faculty of Chemical Engineering, Tarbiat Modares University,Tehran, Iran
2Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran
Potential effects of probiotics on the treatment of several human diseases by modulating the gut microbiota have been discovered. However, the number of viable cells reach the intestine is a great concern. The present study investigates various alginate-based hydrogel biocomposites to immobilize L. plantarum bacteria. This involves chitosan, pectin and gelatin as the coating biopolymers to achieve higher survivability of the cells. Survivability of probiotics were evaluated under simulated gastrointestinal (SGI) conditions with different formulations. Coated alginate hydrogels showed significant (p<0.05) better protection of cells compared to non-coated hydrogel when exposed to SGI conditions. The results also demonstrated better protection of chitosan compared to gelatin and pectin. The structural and physicochemical properties of chitosan-coated alginate hydrogel were analyzed using Scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FTIR).